HS-AFM Movies

Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change
ACS Nanoscience Au, 2022, doi.org/10.1021/acsnanoscienceau.2c00019
Mohit Sharma, Artur P. Biela, Agnieszka Kowalczyk, Kinga Borzęcka-Solarz, Bernard M. A. G. Piette, Szymon Gaweł, Joshua Bishop, Philipp Kukura, Justin L. P. Benesch, Motonori Imamura, Simon Scheuring & Jonathan G. Heddle*

Snf7 spirals sense and alter membrane curvature
Nature Communications, 2022, 13(2174):doi.org/10.1038/s41467-022-29850-z
Nebojsa Jukic#, Alma P. Perrino#, Aurélien Roux & Simon Scheuring*

Chemically induced protein cage assembly with programmable opening and cargo release
Science Advances, 2022, 8(1):eabj9424, doi.org/10.1126/sciadv.abj9424
Izabela Stupka, Yusuke Azuma, Artur P Biela, Motonori Imamura, Simon Scheuring, Elżbieta Pyza, Olga Woźnicka, Daniel P Maskell & Jonathan G Heddle*

Single molecule kinetics of bacteriorhodopsin by HS-AFM
Nature Communications, 2021, 12(7225), doi.org/10.1038/s41467-021-27580-2

Alma P. Perrino, Atsushi Miyagi & Simon Scheuring*

Correlation of Membrane Protein Conformational and Functional Dynamics
Nature Communications, 2021, 12(4363), doi.org/10.1038/s41467-021-24660-1
Raghavendar Reddy Sanganna Gari, Joel José Montalvo‐Acosta, George R Heath, Xiaolong Gao, Crina M Nimigean, Christophe Chipot* & Simon Scheuring*

Localization Atomic Force Microscopy
Nature, 2021, 594(7863):385–390, doi:10.1038/s41586-021-03551-x
George Heath, Ekaterina Kots, Janice Robertson, Shifra Lansky, George Khelashvili, Harel Weinstein & Simon Scheuring*

The hierarchical assembly of septins revealed by high-speed AFM
Nature Communications, 2020, 11(5062), doi.org/10.1038/s41467-020-18778-x
Fang Jiao, Kevin S Cannon, Yi-Chih Lin, Amy S Gladfelter & Simon Scheuring*

Millisecond dynamics of an unlabeled amino acid transporter
Nature Communications, 2020, 11(5016), doi.org/10.1038/s41467-020-18811-z
Tina R Matin#, George R Heath#, Gerard HM Huysmans, Olga Boudker  & Simon Scheuring*

Annexin-A5 Stabilizes Membrane Defects by Inducing Lipid Phase Transition
Nature Communications, 2020, 11(230), doi:10.1038/s41467-019-14045-w
Yi-Chih Lin, Christophe Chipot & Simon Scheuring*

Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity
Science Advances, 2020, 6(5), doi: 10.1126/sciadv.aax8286
Tao Ni#, Fang Jiao#, Xiulian Yu#, Saša Aden, Lucy Ginger, Sophie Williams, Fangfang Bai, Vojta Prazak, Dimple Karia, Phillip Stansfeld, Peijun Zhang, George Munson, Gregor Anderluh, Simon Scheuring*, Robert JC Gilbert*

Real time dynamics of gating-related conformational changes in CorA
eLife, 2019, 8:e47322 doi: 10.7554/eLife.47322
Martina Rangl, Nicolaus Schmandt, Eduardo Perozo* & Simon Scheuring*

Force-induced conformational changes in Piezo1
Nature, 2019, 573(7773):230–234, doi. 10.1038/s41586-019-1499-2
Yi-Chih Lin#, Yusong R Guo#, Atsushi Miyagi, Jesper Levring, Roderick MacKinnon* & Simon Scheuring*

High-Speed AFM Height Spectroscopy (HS-AFM-HS): Microsecond dynamics of unlabeled biomolecules
Nature Communications, 2018, 9(1):4983. doi:10.1038/s41467-018-07512-3
George Heath & Simon Scheuring*

An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel
Nature Communications, 2018, 9(1):3978. doi:10.1038/s41467-018-06414-8.
Arin Marchesi, Xiaolong Gao, Ricardo Adaixo, Jan Rheinberger, Henning Stahlberg, Crina Nimigean* & Simon Scheuring*

Structural titration of receptor ion channel GLIC gating by HS-AFM
PNAS, 2018, doi.org/10.1073/pnas.1805621115
Yi Ruan, Kevin Kao, Solène Lefebvre, Arin Marchesi, Pierre-Jean Corringer Richard K Hite & Simon Scheuring*

High-speed AFM reveals the inner workings of the MinDE protein oscillator
Nano Letters, 2018, 18(1):288–296, doi:10.1021/acs.nanolett.7b04128
Atsushi Miyagi#, Beatrice Ramm#, Petra Schwille & Simon Scheuring*

Lysenin toxin membrane insertion is pH-dependent and non-cooperative
Biophys J, 2017, 113(9):2029-2036, doi.org/10.1016/j.bpj.2017.08.056
Ignacio Munguira#, Hirohide Takahashi#, Ignacio Casuso & Simon Scheuring*

Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis
Nature Cell Biology, 2017, 19(7): Jun 12. doi: 10.1038/ncb3559
Beata Mierzwa#, Nicolas Chiaruttini#, Lorena Redondo-Morata#, Joachim Moser von Filseck, Julia König, Jorge Larios, Ina Poser, Thomas Müller-Reichert, Simon Scheuring, Aurélien Roux* & Daniel Gehrlich*

Dynamin-dependent membrane constriction and fission observed by High-Speed AFM
PNAS, 2017, 114(21):5449-5454. doi: 10.1073/pnas.1619578114
Adai Colom, Lorena Redondo-Morata, Nicolas Chiaruttini, Aurélien Roux* & Simon Scheuring*

Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy
J Mol Biol, 2017, 429(7):977–986; doi.org/10.1016/j.jmb.2017.03.004
Martina Rangl, Luca Rima, Jessica Klement, Atsushi Miyagi, Sandro Keller* & Simon Scheuring*

2017-2 Martina Rangl J Mol Biol-1s

Direct visualization of glutamate transporter elevator mechanism in substrate transport by high-speed AFM
PNAS, 2017, 114(7):1584-1588; doi: 10.1073/pnas.1616413114
Yi Ruan, Atsushi Miyagi, Xiaoyu Wang, Mohamed Chami, Olga Boudker* & Simon Scheuring*

Temperature-controlled high-speed AFM: Real time observation of ripple-phase transitions.
Small, 2016, 12(44):6106-6113
Hirohide Takahashi#, Atsushi Miyagi# Lorena Redondo-Morata & Simon Scheuring*

Automated force controller for amplitude modulation AFM.
Review of Scientific Instruments, 2016, 87(5): doi.org/10.1063/1.4950777
Atsushi Miyagi* & Simon Scheuring*

2016-5 Atsushi Miyagi Review of Scientific Instruments-1s.gif

Real-time visualization of conformational changes upon ligand unbinding from single MloK1 cyclic nucleotide-modulated channels.
Nature Communications, 2016, 7: doi:10.1038/ncomms12789
Martina Rangl, Atsushi Miyagi, Julia Kowal, Henning Stahlberg, Crina Nimigean* & Simon Scheuring*

High-speed atomic force microscopy shows that annexin-V stabilizes membranes on the second timescale.
Nature Nanotechnology, 2016, 11: 783-790
Atsushi Miyagi, Martina Rangl, Christophe Chipot & Simon Scheuring*

Listeriolysin-O membrane damaging activity involves arc formation and lineaction – implication for Listeria monocytogenes escape from phagocytic vacuole.
PLOS Pathogens, 2016, 12(4):e1005597. doi: 10.1371/journal.ppat.1005597
Yi Ruan, Sasa Rezelj, Apolonija Bedina Zavec, Gregor Anderluh* & Simon Scheuring* 

Glass-like membrane protein diffusion in a crowded membrane.
ACS Nano, 2016, 10(2): 2584-2590
Ignacio LB Munguira#, Ignacio Casuso#, Hirohide Takahashi, Felix Rico, Atsushi Miyagi, Mohamed Chami & Simon Scheuring*

Compression of ESCRT-III spiral springs drives membrane deformation.
Cell, 2015, in press
Nicolas Chiaruttini#, Lorena Redondo#, Frédéric Humbert, Martin Lenz*, Simon Scheuring*, Aurélien Roux*

A hybrid high-speed atomic force-optical microscope for visualising single membrane proteins on eukaryotic cells
Nature Communications, 2013, 4: 2155, DOI:10.1038/ncomms3155
Adai Colom#, Ignacio Casuso#, Felix Rico & Simon Scheuring*

High-speed atomic force microscopy: Cooperative adhesion and dynamic equilibrium of junctional microdomain membrane proteins
J Mol Biol, 2012, 423 (2): 249-256
Adai Colom, Ignacio Casuso, Thomas Boudier & Simon Scheuring*

Characterization of the motion of membrane proteins using high speed atomic force microscopy
Nature Nanotechnology, 2012, 7 (8): 525-529
Ignacio Casuso, Jonathan Khao, Mohamed Chami, Perrine Paul-Gilloteaux, Mohamed Husain, Jean-Pierre Duneau, Henning Stahlberg, James N Sturgis & Simon Scheuring* 

Experimental evidence for membrane-mediated protein-protein interaction
Biophys J, 2010, 99 (7): L47-49, doi: 10.1016/j.bpj.2010.07.028
Ignacio Casuso, Pierre Sens, Felix Rico & Simon Scheuring*

Contact-mode high-resolution high-speed atomic force microscopy movies of the purple membrane
Biophys J, 2009, 97 (5): 1354-1361
Ignacio Casuso, Noriyuki Kodera, Christian Le Grimellec, Toshio Ando & Simon Scheuring*